| |
|
Dear customer, please store your samples soon after collection at –80 °c
freezer before submission to the Core Facility. This is especially important for
Catecholamines. In the event, the samples are not stored appropriately,
the Core is not responsible for the outcome of assay results.
Specialized assay services are performed for the bulk of the animal and human research
studies at the Joslin Diabetes Center. Please note that prices have
changed for assay core tests as of June 1 2008.
Routine hormone assays for human and rodent studies include: insulin, C-peptide, leptin, glucagon, ICAM, VCAM, TNF-alpha, C-reactive protein, Interferon-gamma, Interleukins -2, -4, -5 and -10.
Routine metabolite assays for human and rodent studies include: free fatty acids, cortisol, triglycerides and cholesterol.
Please note that, when the total number of samples received is fewer than the capacity of a full kit, we will require an increased turn around time to produce results. In these cases we generally wait until additional samples are received so the entire kit can be used to maintain low prices. Nevertheless, if the results are urgent, and the PI is willing to pay for the entire kit, we will expedite the assay with a quicker turn around. In all cases, we usually wait for 4 weeks (or less) to receive additional samples to use the full kit.
Additional assays performed when requested for specific studies:
Hematology/Immunology: IL-1 alpha, beta, IL-12 P70, IL12/IL23 P40, human ultra-sensitive IL- 12/P70, G-CSF, human ultra-sensitive IL-10,
Metabolites: TGF-beta, OPN, MCP-1, RBP-4 Ghrelin, Free Ghrelin, adiponectin, DAKO insulin, total proinsulin, free insulin, resistin, pancreatic polypeptide, visfatin, adrenaline/noradrenaline, GLP-1, IGF-1 beta, Anti-insulin receptor antibody.
Metabolite General Parameters: HSP70, 8-Isoprostane, C-Reactive Protein, Fox2, GSH, PAI – 1, alpha-Amylase, sCD40L, Serum Amyloid A, Creatinine, Beta-Hydroxybutyrate
Assays for which a ‘wait’ is necessary to insure that the full kit is used:
Adiponectin (mouse)
Glucagon (mouse)
C-peptide (mouse)
Cytokine assays (mouse)
Anti-insulin receptor antibody assay: This is provided for research purposes only, and the turnaround time is around 6 weeks depending on the availability of the radioactive isotope.
Setting up of new assays: If your assays are not on the list mentioned above, please contact Hui Li or Zhen Fu and discuss the possibility of setting it up for your studies.
|
