Learn more about the people and groups that further research at the Joslin Diabetes Center  |  Select an application from the list below:  |  Search JoslinResearch.org  |  Learn about JoslinResearch.org and its features  |  Contact us with questions or to let us know about problems  |  Login to use all our features
Joslin Diabetes Center Website
  Harvard Medical School
Joslin Research Home  
Harvard Medical School Joslin Research Home
 
 
 
  Kahn Lab Home 
   PROTOCOLS
 
 
 
 
Joslin Research Home
Welcome to the Kahn Lab Website -     
  The DERC at Joslin
Protocols:

 Genotyping

  Filename:
Genotyping of IRlox mice by PCR IR-Lox_PCR.pdf
Tail DNA Extraction TailDNA.doc


 Gene Expression

  Filename:
RT-PCR RTPCR_protocol.doc


 DNA Isolation

  Filename:
DNA Mini-Prep (boiling lysis) DNA_MiniPrep.doc
Large-Scale CsCl Plasmid Preparation DNA_CsCl.doc


 Protein Chemistry

  Filename:
Cytosolic MAPK/S6 Kinase Assay CYTOMAPK.doc
HPLC of Iodinated Peptides hplcprep.doc
Purification of Target Proteins (HISTAG) HISTAG.doc
Subcellular Fractionation of Freshly Isolated Rat Fat FATFRAC.doc


 Cell Culture

  Filename:
Brown Fat Cell Isolation BROWN_FAT_CELL_ISOLATION.pdf
Growth and Differentiation of 3T3-L1 and 3T3-F442A Cells 3T3CELLS.doc
Preparation of Adipocytes from Rat Epididymal Fat Pads ADIPISO.doc
Transfection of BOSC 23 cells and infection of 3T3s boscell.doc


 Adenovirus

  Filename:
Adenovirus Amplification Adenovirus_amplification.taniguchi.pdf
Adenovirus Purification Adenovirus_purification.taniguchi.pdf
Adenovirus Titering Adenovirus_Titering.doc


 Westerns

  Filename:
Stripping Western_Blot_Stripping.cypess.doc
Western Buffers CMT_Western_Buffers.cypess.doc
Western Protocol AEP_Western_protocol.cypess.doc


 Serum Assays

  Filename:
Adiponectin ELISA Assay2 Adiponectin.doc
Expression cloning Expression_cloning.doc
GLUCOSE TRANSPORT ASSAY GLUCUPT.doc
GTP-Overlay GTPOVER.doc